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Gibco™ Ham's F-10 Nutrient Mix, HEPES 
Ham's F-10 Nutrient Mixture (F-10) was designed for serum-free growth of Chinese Hamster Ovary (CHO) cells.
Supplier: Gibco™ 12390035
Requires : Supplementation
Description
Ham's F-10 Nutrient Mixture (F-10) was designed for serum-free growth of Chinese Hamster Ovary (CHO) cells. F-10 has since been used for serum-free growth of CHO cultures as well as serum-supplemented growth of other mammalian cells, including COS-7, primary rat astrocytes, and rat prostate epithelial cells.
This DMEM/F-12 is modified as follows:
With: Phenol Red, HEPES, L-glutamine
The complete formulation is available.
Compared to other basal media, F-10 contains a wider variety of components, including zinc, hypoxanthine, and thymidine. Serum-free growth of CHO cells in F-10 has led to a variety of improved formulations, such as Ham's F-12 Nutrient Mixture (F-12).
Using Ham's F-10 Nutrient Mixture
Gibco™ F-10 contains no proteins or growth factors. Therefore, F-10 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). F-10 uses a sodium bicarbonate buffer system (1.2 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.
Order Info
Shipping Condition: Room Temperature
Specifications
| CHO, COS-7, and rat prostate epithelial cells | |
| Animal Origin-free | |
| Liquid | |
| Sterile-filtered | |
| cGMP-compliant under the ISO 13485 standard | |
| 500 mL | |
| Storage conditions: 2-8° C. Protect from light Shipping conditions: Ambient Shelf life: 12 months from date of manufacture |
| Primary Rat Astrocytes | |
| 1 X | |
| Ham's F-10 Nutrient Mixture | |
| High Glucose, Glutamine, HEPES, Phenol Red, Sodium Pyruvate | |
| Gibco™ | |
| Room Temperature |