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Gibco™ DMEM/F-12, powder, HEPES 
DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells.
Supplier: Gibco™ 12400024
Description
DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM/F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. We offer a variety of DMEM/F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This DMEM/F-12 is modified as follows:
With: L-glutamine, HEPES, Phenol Red
Without: Sodium Bicarbonate
The complete formulation is available.
Using DMEM/F-12
DMEM/F-12 is a 1:1 mixture of DMEM and Ham's F-12. This formulation combines DMEM's high concentrations of glucose, amino acids, and vitamins with F-12's wide variety of components. DMEM/F-12 contains no proteins, lipids, or growth factors. Therefore, DMEM/F-12 may require supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM/F-12 uses a sodium bicarbonate buffer system and therefore requires a 5–10% CO2 environment to maintain physiological pH. Powder forms of Gibco™ cell culture media require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).
Specifications
| MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts | |
| Powder | |
| Standard Serum Supplementation | |
| High Glucose, Glutamine, HEPES, Phenol Red, Sodium Pyruvate | |
| cGMP-compliant under the ISO 13485 standard | |
| 10 x 1 L | |
| Storage conditions: 2-8°C Shipping conditions: Ambient Shelf life: 36 months from date of manufacture |
| Animal Origin-free | |
| DMEM (Dulbecco's Modified Eagle Medium)/F-12 | |
| Sterile-filtered | |
| No Sodium Bicarbonate | |
| Gibco™ | |
| Room Temperature |