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Gibco™ DMEM/F-12, powder 
Dulbecco's Modified Eagle Medium:Nutrient Mixture F-12 (DMEM / F-12) is a widely used basal medium for supporting the growth of many different mammalian cells.
Supplier: Gibco™ 12500062
Description
Dulbecco's Modified Eagle Medium:Nutrient Mixture F-12 (DMEM / F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM / F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. We offer a variety of Gibco™ DMEM / F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This DMEM / F-12 is modified as follows:
With: L-glutamine, Phenol Red
Without: HEPES, Sodium Bicarbonate
The complete formulation is available.
Gibco™ DMEM / F-12 is a 1:1 mixture of DMEM and Ham's F-12. This formulation combines DMEM's high concentrations of glucose, amino acids and vitamins with F-12's wide variety of components.
Using DMEM/F-12
DMEM / F-12 contains no proteins, lipids, or growth factors. Therefore, DMEM / F-12 may require supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM / F-12 uses a sodium bicarbonate buffer system and therefore requires a 5-10% CO2 environment to maintain physiological pH.
Powder forms of Gibco™ cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).
Specifications
| MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts | |
| Powder | |
| Standard Serum Supplementation | |
| High Glucose, Glutamine, Phenol Red, Sodium Pyruvate | |
| cGMP-compliant under the ISO 13485 standard | |
| 10 x 1 L | |
| Storage conditions: 2-8° C Shipping conditions: Ambient Shelf life: 36 months from date of manufacture |
| Animal Origin-free | |
| DMEM (Dulbecco's Modified Eagle Medium)/F-12 | |
| Sterile-filtered | |
| No HEPES | |
| Gibco™ | |
| Room Temperature |