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Invitrogen™ Human FGF6 ELISA Kit 
ELISA
$866.00 - $866.00
Specifications
| Accession Number | P10767 |
|---|---|
| Assay Range | 8.23 to 6000 pg/mL |
| Assay Sensitivity | 6 pg/mL |
| Calibration Range | 6 pg/mL to 6000 pg/mL |
| Assay | Solid-phase sandwich ELISA |
Description
The Human Fibroblast Growth Factor 6 (Hu FGF6) ELISA quantitates Hu FGF6 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu FGF6. Principle of the method The Human FGF6 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
FGF-6 is a secreted, heparin-binding growth factor that is a member of the FGF family. Proteins of this family play a central role during prenatal development, postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF-6 is expressed in leukemia cell lines with platelet megakaryocytic differentiation potential. It signals through FGFR 1c, 2c, and 4.4Specifications
| P10767 | |
| 6 pg/mL | |
| Solid-phase sandwich ELISA | |
| ELISA | |
| Plasma, Serum, Supernatant | |
| Human | |
| Colorimetric Microplate Reader | |
| HBGF-6,HST2 | |
| <10 | |
| HRP | |
| Angiogenesis | |
| 2°C to 8°C | |
| FGF6 | |
| 4 hrs 45 mins |
| 8.23 to 6000 pg/mL | |
| 6 pg/mL to 6000 pg/mL | |
| HRP | |
| Growth Factors & Receptors | |
| This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF. | |
| ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis | |
| 2251 | |
| <12% | |
| Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers | |
| RUO | |
| Plasma 50 μL, Serum 50 μL, Supernatant 100 μL | |
| HBGF-6, HST2 | |
| 1 hrs 20 mins |