Invitrogen™ F(ab')2-Goat anti-Rabbit IgG (H+L) Secondary Antibody, FITC, eBioscience™

Description

Description: The anti-rabbit IgG polyclonal antibody reacts with the heavy and light chains of rabbit IgG, with minimal cross reaction to human, mouse and rat serum proteins. Reactions to non-immunoglobulin rabbit serum proteins have not been detected. Applications Reported: This polyclonal antibody has been reported for use in flow cytometric analysis, immunohistochemical staining, and immunocytochemistry. Applications Tested: This polyclonal antibody has been tested by flow cytometric analysis and immunocytochemistry to detect polyclonal rabbit antibodies. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. For immunocytochemistry applications, this can be used at less than or equal to 10 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser. Filtration: 0.2 μm post-manufacturing filtered.

Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.