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MilliporeSigma™ EZ-Magna ChIP™ HT96 Chromatin Immunoprecipitation Kit
Reliable streamlined approach works for both cells and tissues and can be performed using either multi-channel pipette or automated liquid handling instrument
Supplier: MilliporeSigma™ 1710078
Includes:
- Magna ChIP protein A/G magnetic beads
- HT96 Nuclei Isolation Buffer
- HT96 ChIP Buffer
- Low Stringency IP Wash Buffer
- HT96 ChIP Elution Buffer
- Proteinase K Solution
- Protease Inhibitor Cocktail III
- 10X Glycine
- 10X PBS
- 96 Well ChIP Plate
- 96 Well Thermal Plate
- Plate Seal
- Strip Caps
- Anti-Trimethyl-Histone H3 (Lys4) -positive control antibody
- Normal Rabbit IgG -negative control antibody
- GAPDH Primers - PCR positive control
Requires : Magna Grip HT96 Rack 96-well magnetic separation stand
Description
- Complete set of materials for up to 96 ChIP reactions
- Protein A+G bead blend for ChIP with a broader range of antibodies than A or G alone
- Low Chromatin requirements: 10, 000 to 100, 000 cells per reaction
- Includes negative and positive control antibodies and control primer set to simplify validation of experimental procedure
- Optimized streamlined protocol with only a single buffer for sonication, IP, or wash; and protocols for automated liquid handling systems
- Protocols for using cells or tissues
- Direct analysis of DNA without additional clean-up steps
- Compatible with ChIPAb+ validated antibody and primer sets
Specifications
| store components at the temperatures indicated on the labels | |
| Allows the performance of chromatin Immunoprecipitation in a 96-well plate-based format | |
| 96 Assays | |
| Kit components are stable for 1 year from date of shipment when stored as directed |