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Gibco™ Expi293™ Expression Medium 
Expi293 Expression Medium designed for growth and transfection of suspension-adapted human embryonic kidney (HEK) 293 cells.
Supplier: Gibco™ A1435101
Description
Expi293 Expression Medium is a chemically defined, serum-free, protein-free medium for growth and transfection of suspension-adapted human embryonic kidney (HEK) 293 cells. It is specifically designed as a core component to support high density culture of Expi293F cells in the Expi293 Expression System for scalable transient protein expression.
- Supports growth of suspension 293 cultures to densities of over 1.5 x 10^7 cells/mL
- Transfection compatible serum-free suspension culture medium enables transfection efficiencies of greater than 70% using ExpiFectamine transfection reagent
- Enables sustained, high level expression of high density transiently transfected cultures, achieving yields of up to 1 gram per liter of recombinant protein
- Supports growth and transfection of 293 cells in culture formats of less than 1 mL in multiwell plates to greater than 10 L in disposable bioreactors
- Does not contain phenol red
Expi293 Expression Medium is formulated with GlutaMAX-I reagent. It is ready to use, with no supplementation required. The chemically defined formulation results in high reproducibility and lot-to-lot reliability. Expi293 Expression Medium contains no human or animal-origin components. The medium is not recommended for adherent cell cultures.
Specifications
| 293E (HEK), 293 (HEK), Expi293F™, FreeStyle™ 293-F Cells, 293F | |
| Animal Origin-free, Chemically-defined, Protein-free, Serum-free | |
| Liquid | |
| Serum-free | |
| Sterile-filtered | |
| No HEPES, No Phenol Red, No Hypoxanthine, No Thymidine, No Insulin, No Vitamin A, No 2-Mercaptoethanol | |
| 1 L | |
| < 1 EU/mL | |
| No antibiotics | |
| 275-295 mOsm/kg | |
| Store in the dark at +2°C to +8°C | |
| Suspension Cell Culture |
| Embryonic Kidney Cells | |
| 1 X | |
| Expression Medium | |
| Sterile | |
| High Glucose, GlutaMAX, Sodium Pyruvate, Sodium Bicarbonate, Dextrose, Pyridoxine HCl | |
| Expi293™ | |
| Room Temperature | |
| Bottle(s) | |
| Calcium, Magnesium, Sodium Phosphate | |
| Cell Culture Grade | |
| CO2 | |
| Expi293F™ |
For Research use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.