MilliporeSigma™ pT7Blue T-Vector

Description

Many thermostable DNA polymerases, such as Taq DNA polymerase, leave single 3' A-nucleotide overhangs on their reaction products. These molecules can be ligated directly to a vector containing compatible single T-nucleotide overhangs. The pT7Blue vector has been specifically constructed for this application and has been prepared for T-cloning by digestion with EcoRV followed by the addition of single 3' dT residues at each end.