MilliporeSigma™ pET-31b(+) DNA Vector

Description

The pET-31b(+) vector is designed for cloning and high-level expression of peptide sequences fused with the 125aa ketosteroid isomerase protein (1). The unique AlwN I cloning site allows the unidirectional insertion of of tandemly repeated peptide coding regions separated by methionine codons. The sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the vector map.