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MilliporeSigma™ Novagen™ BL21(DE3) Competent Cells
Achieve greater efficiency, reproducibility and convenience with Novagen prepared competent cells. BL21(DE3) is a chemically competent E. coli cell suitable for transformation and high-level protein expression using a T7 RNA polymerase-IPTG induction system.
Supplier: MilliporeSigma™ 694504
Description
- Deficient in Ion and ompT proteases, BL21 and its derivatives are ideal for many applications
T7 expression strain - Lysogen of bacteriophage DE3
- Carries copy of T7 RNA polymerase gene under control of the lacUV5 promoter
- Suitable for production of protein from target genes cloned in appropriate T7 expression vectors, using IPTG as an inducer
Specifications
| Guaranteed efficiency: >2 x 107 cfu/μg | |
| Store at ≤-70deg;C, Avoid repeated freeze/thaw | |
| 1 mL (5 x 0.2 mL aliquots) |