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  8. 4304134

Applied Biosystems™ TaqMan™ Reverse Transcription Reagents DFS Item

TaqMan Reverse Transcription Reagents provide the necessary reagents for reverse transcriptase-PCR (RT-PCR). Random hexamers, oligo (dT)16, and reverse primers are included for cDNA synthesis flexibility.

Supplier:  Applied Biosystems™ 4304134

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Catalog No. 4304134

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Description

Description

TaqMan Reverse Transcription Reagents provide the necessary reagents for reverse transcriptase-PCR (RT-PCR). Random hexamers, oligo (dT)16, and reverse primers are included for cDNA synthesis flexibility. These reagents are designed for reverse transcription and PCR amplification of a specific target RNA from either total RNA or mRNA. The reagents in this kit facilitate two-step RT-PCR, which is performed in two separate reactions and is useful when detecting multiple transcripts from a single cDNA reaction or when storing a portion of the cDNA.

Advantages of TaqMan Reverse Transcription Reagents

  • Contains MultiScribe Reverse Transcriptase, a recombinant Moloney murine leukemia virus (MMLV) reverse transcriptase
  • Includes random hexamers and oligo (dT)16 for flexibility
  • Individual components provide versatility in assay setup
  • Compatible with two-step RT-PCR reactions

Using TaqMan Reverse Transcription Reagents

  • TaqMan Reverse Transcription Reagents provide all the necessary components for reverse transcription of RNA to cDNA
  • For two-step RT-PCR, random hexamers, oligo (dT)16, and sequence-specific reverse primers can all be used
  • The choice of primers for reverse transcription is best made after experimentally evaluating all three priming systems
Specifications

Specifications

50 U/μL
Kit
42°C
MultiScribe
Dry Ice
First-Strand cDNA
Separate components
Up to 5 kb

Includes 10 units. Each unit contains:

• MultiScribe Reverse Transcriptase, 100 μL (50 U/μL)
• RNase Inhibitor, 100 μL (20 U/μL)
• dNTP Mixture, 1 mL (2.5 mM each dNTP)
• Oligo (dT)16, 100 μL (50 μM)
• Random Hexamers, 100 μL (50 μM)
• 10X RT Buffer, 1.5 mL
• MgCl2 Solution, 1.5 mL

Store at –25°C to –15°C.
Reverse Transcription
2000 Reactions
Yes
Real Time PCR (qPCR)
2000 Reactions at 10 μL
Reverse Transcription
RNA
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For Research Use Only. Not for use in diagnostic procedures.