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Invitrogen™ mMESSAGE mMACHINE™ T7 Transcription Kit 
in vitro synthesis of large amounts of capped RNA
Supplier: Invitrogen™ AM1344
Description
mMESSAGE mMACHINE™ kits are designed for the in vitro synthesis of large amounts of capped RNA. Capped RNA mimics most eukaryotic mRNAs found in vivo, because it has a 7-methyl guanosine cap structure at the 5′ end.
- RNA Yield: 20-30μg
- Include cap analog [m7G(5′)ppp(5′)G] in ultra high-yield transcription reaction
- Simplified reaction format in which all four ribonucleotides and cap analog are mixed in single solution
- Cap analog: GTP ratio of solution is 4:1, which is optimal for maximizing both RNA yield and the proportion of capped transcripts
- For routine synthesis of capped RNAs for oocyte microinjection, in vitro translation, transfection, and other applications
- High yields are achieved by optimizing reaction conditions for RNA synthesis in presence of high nucleotide concentrations
- RNA synthesized is protected from degradation by contaminating ribonucleases that present with RNase inhibitor - component of the Enzyme Mix
Specifications
| T7 Enzyme Mix, 10X Reaction Buffer 2XNTP⁄CAP Solution, GTP Solution, pTRI-Xef, TURBO DNase, Ammonium Acetate Stop Solution, Lithium Chloride Precipitation Solution, and Gel Loading Buffer II are all stored at –20°C. Nuclease-free Water may be stored at any temperature. | |
| Kit | |
| In Vitro Transcription, synthetic mRNA, mRNA, transfection, LNPs, GENEart, linearized DNA plasmid, delivery, PCR products | |
| Ambion™, mMESSAGE mMACHINE™ | |
| 25 Reactions | |
| Dry Ice | |
| PCR Products, Linearized DNA plasmid |
| Synthetic mRNA, mRNA | |
| mRNA, Synthetic mRNA | |
| 25 Reactions | |
| T7 In Vitro Transcription kit | |
| T7 | |
| 20-30 μg |
For Research Use Only. Not for use in diagnostic procedures.