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5,5'-Dithiobis(2-nitrobenzoic acid), 99%
CAS: 69-78-3 | C14H8N2O8S2 | 396.34 g/mol
Supplier: Thermo Scientific Chemicals A1433114
Description
Reagent for determination of sufhydryl groups.5,5'-dithiobis-(2-nitrobenzoic acid) is widely utilized as a non-fluorescent probe to quantify the number or the concentration of thiol groups in a sample. It acts as a cleavable cross-linking agent. It finds an important application as a sensitive reagent for determining free sulfhydryl content in proteins, peptides, and tissues. It is also employed in high-performance liquid chromatography (HPLC) to determine the alkylthiol content.
This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.
ApplicationsReagent for determination of sufhydryl groups.5,5′-dithiobis-(2-nitrobenzoic acid) is widely utilized as a non-fluorescent probe to quantify the number or the concentration of thiol groups in a sample. It acts as a cleavable cross-linking agent. It finds an important application as a sensitive reagent for determining free sulfhydryl content in proteins, peptides, and tissues. It is also employed in high-performance liquid chromatography (HPLC) to determine the alkylthiol content.
Solubility
Soluble in water and ethanol.
Notes
Keep the container tightly closed in a dry and well-ventilated place. Incompatible with strong oxidizing agents and strong bases.
Specifications
5,5'-Dithiobis(2-nitrobenzoic acid) | |
∼240°C (decomposition) | |
MFCD00007140 | |
1896821 | |
Soluble in water and ethanol. | |
OC(=O)C1=CC(SSC2=CC=C(C(=C2)C(O)=O)[N+]([O-])=O)=CC=C1[N+]([O-])=O | |
396.34 | |
CHEBI:86228 | |
99% |
69-78-3 | |
C14H8N2O8S2 | |
25 g | |
dtnb, 5,5'-dithiobis 2-nitrobenzoic acid, ellman's reagent, 3-carboxy-4-nitrophenyl disulfide, dithionitrobenzoic acid, dithiobisnitrobenzoic acid, 5,5'-disulfanediylbis 2-nitrobenzoic acid, benzoic acid, 3,3'-dithiobis 6-nitro, 3,3'-dithiobis 6-nitrobenzoic acid, 5,5'-dithio-bis 2-nitrobenzoic acid | |
KIUMMUBSPKGMOY-UHFFFAOYSA-N | |
5-[(3-carboxy-4-nitrophenyl)disulfanyl]-2-nitrobenzoic acid | |
6254 | |
396.36 |
Chemical Identifiers
69-78-3 | |
396.34 | |
KIUMMUBSPKGMOY-UHFFFAOYSA-N | |
6254 | |
5-[(3-carboxy-4-nitrophenyl)disulfanyl]-2-nitrobenzoic acid |
C14H8N2O8S2 | |
MFCD00007140 | |
dtnb, 5,5'-dithiobis 2-nitrobenzoic acid, ellman's reagent, 3-carboxy-4-nitrophenyl disulfide, dithionitrobenzoic acid, dithiobisnitrobenzoic acid, 5,5'-disulfanediylbis 2-nitrobenzoic acid, benzoic acid, 3,3'-dithiobis 6-nitro, 3,3'-dithiobis 6-nitrobenzoic acid, 5,5'-dithio-bis 2-nitrobenzoic acid | |
CHEBI:86228 | |
OC(=O)C1=CC(SSC2=CC=C(C(=C2)C(O)=O)[N+]([O-])=O)=CC=C1[N+]([O-])=O |
Safety and Handling
GHS H Statement
H315-H319-H335
Causes skin irritation.
Causes serious eye irritation.
May cause respiratory irritation.
P261-P264b-P271-P280-P302+P352-P304+P340-P305+P351+P338-P312-P332+P313-P362-P501c
H315-H319-H335
EINECSNumber : 200-714-4
RTECSNumber : DG9650000
TSCA : Yes
Recommended Storage : Ambient temperatures
RUO – Research Use Only