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3,3',5,5'-Tetramethylbenzidine soln., Ready-to-Use, high sensitivity, Thermo Scientific Chemicals
3,3â,5,5â-Tetramethylbenzidine (TMB, CAS # 54827-17-7) is a blue chromogenic substance used in staining techniques such as immunohistochemistry.
$363.30 - $876.93
Chemical Identifiers
| CAS | 54827-17-7 |
|---|---|
| Molecular Formula | C16H20N2 |
| Molecular Weight (g/mol) | 240.35 |
| MDL Number | MFCD00007748 |
| InChI Key | UAIUNKRWKOVEES-UHFFFAOYSA-N |
| Synonym | 3,3',5,5'-tetramethylbenzidine, 3,3',5,5'-tetramethyl-1,1'-biphenyl-4,4'-diamine, 3,5,3',5'-tetramethylbenzidine, tmb, 3,3',5,5'-tetramethyl benzidine, tmb substrate, bm blue, ccris 4727, tmb plus, tmb, insoluble |
| PubChem CID | 41206 |
| IUPAC Name | 4-(4-amino-3,5-dimethylphenyl)-2,6-dimethylaniline |
| SMILES | CC1=CC(=CC(=C1N)C)C2=CC(=C(C(=C2)C)N)C |
| Catalog Number | Mfr. No. | Quantity | Price | Quantity | |||||
|---|---|---|---|---|---|---|---|---|---|
| Catalog Number | Mfr. No. | Quantity | Price | Quantity | |||||
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AAJ61325EQE
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Thermo Scientific Chemicals
J61325EQE |
125 mL |
Each for $363.30
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AAJ61325AP
|
Thermo Scientific Chemicals
J61325AP |
500 mL |
Each for $576.27
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AAJ61325AU
|
Thermo Scientific Chemicals
J61325AU |
1000 mL |
Each for $876.93
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Description
TMB is a substrate for horseradish peroxidase. Recommended for ELISA (microwell) procedures,3,3?,5,5?-Tetramethylbenzidine has been used in a study to demonstrate that by measuring substrate absorption values off the absorption maximum, the measuring range of any immunoenzymometric assay can be extended significantly without jeopardizing assay characteristics. 3,3?,5,5?-Tetramethylbenzidine has also been used in a study to understand the formation of CuS concave superstructures with peroxidase-like activity.
This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.
General Description
• TMB is an aromatic amine that acts as a hydrogen donor in horseradish peroxidase-catalyzed reactions
• The transfer of hydrogen results in a blue stain associated with the target of interest
• This pH-dependent color change can be confirmed using spectrophotometric methods
• TMB is commonly used in immunohistochemistry; TMB is light-sensitive, and all applications should be performed in the dark
Application
• TMB is suitable for immunohistochemistry and immunocytochemistry applications including ELISA and cell and tissue staining
Chemical Identifiers
| 54827-17-7 | |
| 240.35 | |
| UAIUNKRWKOVEES-UHFFFAOYSA-N | |
| 41206 | |
| CC1=CC(=CC(=C1N)C)C2=CC(=C(C(=C2)C)N)C |
| C16H20N2 | |
| MFCD00007748 | |
| 3,3',5,5'-tetramethylbenzidine, 3,3',5,5'-tetramethyl-1,1'-biphenyl-4,4'-diamine, 3,5,3',5'-tetramethylbenzidine, tmb, 3,3',5,5'-tetramethyl benzidine, tmb substrate, bm blue, ccris 4727, tmb plus, tmb, insoluble | |
| 4-(4-amino-3,5-dimethylphenyl)-2,6-dimethylaniline |
Specifications
| 54827-17-7 | |
| C16H20N2 | |
| 125 mL | |
| 2808541 | |
| Insoluble in water. Soluble in organic solvents such as DMSO,96 % Ethanol,Acetone,Chloroform,Toluene. | |
| CC1=CC(=CC(=C1N)C)C2=CC(=C(C(=C2)C)N)C | |
| 240.35 | |
| 240.35 | |
| 3,3',5,5'-Tetramethylbenzidine soln., Ready-to-Use, high sensitivity |
| Yellow | |
| MFCD00007748 | |
| UN2810 | |
| 3,3',5,5'-tetramethylbenzidine, 3,3',5,5'-tetramethyl-1,1'-biphenyl-4,4'-diamine, 3,5,3',5'-tetramethylbenzidine, tmb, 3,3',5,5'-tetramethyl benzidine, tmb substrate, bm blue, ccris 4727, tmb plus, tmb, insoluble | |
| UAIUNKRWKOVEES-UHFFFAOYSA-N | |
| 4-(4-amino-3,5-dimethylphenyl)-2,6-dimethylaniline | |
| 41206 | |
| Liquid |
Safety and Handling
GHS H Statement
H370-H312-H332
Causes damage to organs.
Harmful in contact with skin.
Harmful if inhaled.
P210-P233-P235-P240-P241-P242-P243-P260-P264b-P270-P271-P280-P301+P312-P303+P361+P353-P304+P340-P307+P311-P311-P312-P330-P363-P370+P378q-P501c
H226-H302+H312-H331-H336-H370
DOTInformation : Transport Hazard Class: 6.1; Packing Group: III; Proper Shipping Name: TOXIC, LIQUIDS, ORGANIC, N.O.S.
EINECSNumber : 259-364-6
TSCA : Yes
Recommended Storage : Keep cold
RUO – Research Use Only