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General Western Blot Reagents

Specially formulated reagents for a variety of analytical western blot processes; includes chromogenic and chemiluminescent kits and substrates, transfer solutions, enhancing buffers, and stripping buffers for reprobing under alternate conditions.
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1 – 15 of 44 results
1

Thermo Scientific™ TMB Substrate Solution

A ready-to-use substrate system containing 3.3', 5.5'-tetramethylbenzidine (TMB)

2

Thermo Scientific™ Restore™ Western Blot Stripping Buffer

Safely and effectively remove primary and secondary antibodies from nitrocellulose and PVDF membranes to allow chemiluminescent Western blots to be reprobed.

Content And Storage Storage: Upon receipt store at room temperature or 4°C. Product shipped at ambient temperature.
Form Liquid
Product Line Restore™
3

Thermo Scientific™ Restore™ PLUS Western Blot Stripping Buffer

Remove high-affinity antibodies from membranes so they can be reprobed and detected with chemiluminescent substrates.

Content And Storage Upon receipt store at room temperature. Product shipped at ambient temperature.
Form Liquid
Product Line Restore™
4
Promotions Available

Invitrogen™ Bolt™ Antioxidant  DFS Item

A proprietary reagent that maintains proteins in a reduced state during protein gel electrophoresis

5

MilliporeSigma™ Calbiochem™ SeaBlock™ Reagent, Salmon Plasma

Ready to use blocking reagent for western blotting immunoassays.

6

MilliporeSigma™ Calbiochem™ SignalBoost™ Immunoreaction Enhancer Kit

Designed to enhance desired signal in assays such as immunoblotting, dot blotting, ELISA.

7

MilliporeSigma™ Calbiochem™ MOWIOL™ 4-88 Reagent

Used for mounting cells on slides for detection of cell-surface antigens.

8

MilliporeSigma™ ReBlot Plus Kit and Reagents

Blots can be stripped of antibodies in approximately 15 minutes at room temperature

For Use With (Application) Western Blot
9

Invitrogen™ NuPAGE™ MES SDS Buffer Kit (for Bis-Tris Gels)  DFS Item

Designed for separation of small- to medium-size proteins on NuPAGE Novex Bis-Tris gels

10

Thermo Scientific™ Pierce™ 1-Step Transfer Buffer

Perform rapid semi-dry transfer of 10-300kDa proteins from polyacrylamide gels (SDS-PAGE) to nitrocellulose or PVDF membranes using the Pierce G2 Fast Blotter.

Product Type Transfer Buffer
11

Invitrogen™ XCell II™ Blot Module  DFS Item

Easy transfer of proteins or nucleic acids from mini-gels to membranes

12

Thermo Scientific™ Restore™ Fluorescent Western Blot Stripping Buffer

Quickly and gently remove primary and near-infrared (IR) dye-labeled secondary antibodies from Western blots.

Content And Storage Upon receipt store at room temperature.
Form Liquid
Product Line Restore™
13

Invitrogen™ NuPAGE™ MOPS SDS Buffer Kit (for Bis-Tris Gels)  DFS Item

All buffers are made with high-purity reagents and are strictly quality controlled

14

Thermo Scientific™ SuperSignal™ Western Blot Enhancer

Increase both signal intensity and sensitivity 3- to 10-fold with this membrane treatment reagent and primary antibody diluent.

Content And Storage Upon receipt store solutions at 4°C Products are shipped at ambient temperature.
Form Liquid
Product Type Western Blot Enhancer
Membrane Compatibility Nitrocellulose, PVDF
For Use With (Application) Western Blot
Product Line SuperSignal™
Detection Method Chemiluminescence, Fluorescence, Colorimetric
15

Thermo Scientific™ Pierce™ Western Blot Signal Enhancer

Greatly enhance the effectiveness of primary antibodies and intensify the final Western blot detection signal with this two-reagent blot conditioning system.