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Search results for "competent E. coli"

1 – 15 of 130 results
2

Invitrogen™ One Shot™ TOP10 Chemically Competent E. coli  DFS Item

One Shot TOP10 Chemically Competent E. coli are ideal for high-efficiency cloning and plasmid DNA propagation and are provided at a transformation efficiency of 1 x 109 cfu/μg plasmid DNA.

Shipping Condition Dry Ice
Blue/White Screening Yes (lacZΔM15)
Cloning Methylated DNA Yes (mcrA)
Transformation Efficiency Level High Efficiency (>1 x 109 cfu/μg)
High-throughput Compatibility Low
Improves Plasmid Quality Yes (endA1)
Preparing Unmethylated DNA No
Plasmid High Copy Plasmid
Reduces Recombination Yes (recA1)
T1 Phage - Resistant (tonA) No
Antibiotic Resistance Bacterial Yes (Streptomycin)
Format Tube
Product Type Chemically Competent Cells
Species E. coli (K12)
Product Line One Shot™
Contains F' Episome No
3

Invitrogen™ One Shot™ BL21(DE3)pLysS Chemically Competent E. coli  DFS Item

One Shot BL21(DE3)pLysS Chemically Competent E. coli cells are ideal for use with bacteriophage T7 promoter-based expression systems such as pRSET and pET.

Shipping Condition Dry Ice
Blue/White Screening No
Cloning Methylated DNA No
Transformation Efficiency Level Medium Efficiency (1 x 108 to 1 x 109 cfu/μg)
ToxicProteins No
High-throughput Compatibility Low
Improves Plasmid Quality No
ImprovesProteinStability Yes (lon, ompT)
Promoter T7
Preparing Unmethylated DNA Yes (dcm)
Reduces Recombination No
ImprovesRNAStability Yes (pLysS)
T1 Phage - Resistant (tonA) No
Antibiotic Resistance Bacterial Yes (Chloramphenicol)
Format Tube
Product Type Chemically Competent Cells
Species E. coli (B)
Product Line One Shot™
Contains F' Episome No
4

Invitrogen™ MultiShot™ StripWell TOP10 Chemically Competent E. coli  DFS Item

MultiShot StripWell TOP10 Chemically Competent E. coli cells are ideal for high-efficiency cloning and plasmid propagation, achieving a transformation efficiency of >1 x 108 cfu/μg with control plasmid DNA in StripWell format.

Shipping Condition Dry Ice
Blue/White Screening Yes (lacZΔM15)
Cloning Methylated DNA Yes (mcrA)
Transformation Efficiency Level Medium Efficiency (1 x 108 to 1 x 109 cfu/μg)
High-throughput Compatibility High
Improves Plasmid Quality Yes (endA1)
Preparing Unmethylated DNA No
Plasmid High Copy Plasmid
Reduces Recombination Yes (recA1)
T1 Phage - Resistant (tonA) No
Antibiotic Resistance Bacterial Yes (Streptomycin)
Product Type Chemically Competent Cells
Product Line MultiShot™
Contains F' Episome No
5

Invitrogen™ BL21 Star™ (DE3)pLysS One Shot™ Chemically Competent E. coli  DFS Item

One Shot BL21 Star (DE3)pLysS Chemically Competent E. coli are cells designed for applications that require high-level expression of non-toxic recombinant proteins from low or high copy number vectors, such as pRSET and pET.

6

Thermo Scientific™ DH10B Competent Cells; High Efficiency

High efficiency, chemically competent E. coli cells. The DH10B strain is suitable for cloning DNA containing methylcytosine, 5-hydroxymethylcytosine, and methyladenine, allowing both prokaryotic and eukaryotic genomic DNA to be cloned efficiently.

7

Invitrogen™ Competent Cell Sampler  DFS Item

The Competent Cell Sampler Kit contains a pair of four different strains of chemically competent E. coli cells in One Shot format.

8

Thermo Scientific™ DH5α Competent Cells

Chemically competent E. coli cells recommended for routine subcloning into plasmid vectors. Subcloning efficiency cells are not suitable for the generation of cDNA libraries.

9

New England Biolab Ltd dam-/dcm- Competent E. coli 6 x 0.2 ml/tube  DFS Item

Methyltransferase deficient chemically competent E. coli cells suitable for growth of plasmids free of Dam and Dcm methylation. Note that dam- strains are not recommended as a host for primary cloning/ligation. The dam mutation can result in an increased mutation rate in the cell and a reduction in the transformation efficiency. DNA should be maintained in a dam+ strain unless there is a specific need for DNA free of Dam or Dcm methylation.

10

MilliporeSigma™ Novagen™ Rosetta™ (DE3) Competent Cells

Rosetta host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli.

Content And Storage ≤-70°C
11

Invitrogen™ TOPO™ XL-2 Complete PCR Cloning Kit, with One Shot™ OmniMAX™ 2 T1R Chemically Competent E. coli Cells  DFS Item

Provides all the necessary elements for efficient cloning of extra-long PCR products (up to 13 kb)

Shipping Condition Approved for shipment at Room Temperature or on Wet or Dry Ice
Promoter T3, T7
Content And Storage Box 1: Store at -5 to -30°C.
• pCR XL-2-TOPO vector
• Salt Solution
• T3 Primer
• T7 Primer
• XL Control PCR Template
• XL Control PCR Primers
• Platinum SuperFi Green PCR Master Mix
• SuperFi GC Enhancer
• Nuclease-free water

Box 2: Store at room temperature.
• Gel Solubilization Buffer (L3)
• Binding Buffer (B2)
• Wash Buffer (W1)
• Elution Buffer (E1)
• PureLink Clean-up Spin Columns (in Wash Tubes)
• PureLink Recovery/Elution Tubes

Box 3:
• One Shot OmniMAX 2 T1R chemically competent E. coli
• S.O.C. Medium
• pUC 19 Control DNA

Store competent cells and pUC19 at -65 to -85°C.
Antibiotic Resistance Bacterial Ampicillin (AmpR), Kanamycin (KanR)
Format Kit
Product Type Cloning Kit
Cloning Method Zero Blunt TOPO
Bacterial or Yeast Strain OmniMAX2
For Use With (Application) PCR Cloning
Vector pCR-XL-2-TOPO
Product Line TOPO™
Cell Type Chemically Competent E. coli
12

Invitrogen™ One Shot™ MAX Efficiency™ DH5α-T1R Competent Cells  DFS Item

One Shot MAX Efficiency DH5α-T1R Competent Cells are derived from the popular DH5α strain and carry the tonA (fhuA) mutation that confers resistance to T1 and T5 phage.

13

Invitrogen™ One Shot™ TOP10F' Chemically Competent E. coli  DFS Item

One Shot TOP10F' Chemically Competent E. coli are identical to TOP10 cells, with the addition of an F' episome.

Shipping Condition Dry Ice
Blue/White Screening Yes (lacZΔM15)
Cloning Unstable DNA Not suitable for cloning unstable DNA
Cloning Methylated DNA Yes (mcrA)
Transformation Efficiency Level High Efficiency (>1 x 109 cfu/μg)
High-throughput Compatibility Low
Improves Plasmid Quality Yes (endA1)
Promoter Trc, Tac, Lac
Preparing Unmethylated DNA No
Reduces Recombination Yes (recA1)
T1 Phage - Resistant (tonA) No
Antibiotic Resistance Bacterial Yes (Streptamycin, Tetracycline)
Format Tube
Product Type Chemically Competent Cells
Species E. coli (K12)
Product Line One Shot™
Contains F' Episome Yes
14

Invitrogen™ MC1061/P3 Chemically Competent E. coli  DFS Item

MC1061/P3 Chemically Competent E. coli are used for highly efficient transformations that require the P3 episome for selection and maintenance of vectors encoding the tyrosine tRNA suppressor (synthetic supF gene, such as pCDM8, pcDNA1.1, or any other supF-containing vector).

15

Invitrogen™ MultiShot™ TOP10 Chemically Competent E. coli  DFS Item

MultiShot TOP10 Chemically Competent E. coli cells are derived from DH10B and are one of the most popular laboratory strains.