Miscellaneous Molecular Biology Reagents
Invitrogen™ EDTA (0.5 M), pH 8.0, RNase-free
Invitrogen Molecular biology grade, 0.5 M EDTA, pH 8.0 solution is supplied in one bottle. The solution is certified RNase-free, economical, and ready-to-use.
Invitrogen™ UltraPure™ BSA (50 mg/mL)
Ambion Ultrapure BSA is a 'non-acetylated' BSA pure enough to use when DNA or RNA integrity is critical.
Thermo Scientific™ DTT (dithiothreitol)
Stabilize enzymes and other proteins which possess free sulfhydryl groups with DTT, shown to restore activity lost by oxidation of these groups in vitro.
Thermo Scientific™ X-Gal Solution, ready-to-use
Effectively screen colonies with X-Gal, an inert chromogenic substrate for beta-galactosidase which hydrolyzes X-Gal, forming an intense blue precipitate.
Invitrogen™ UltraPure™ Phenol:Chloroform:Isoamyl Alcohol (25:24:1, v/v)
UltraPure™ Phenol:Chloroform:Isoamyl Alcohol (25:24:1, v/v) is used in the purification of nucleic acids. This reagent consists of highly pure chloroform, isoamyl alcohol, and UltraPure™ Phenol saturated with Tris-HCl.
Invitrogen™ UltraPure™ Glycerol
UltraPure Glycerol is suitable for the concentration and storage of enzymes at low temperatures. A 50% (v/v) solution will not freeze at -20°C.
Invitrogen™ UltraPure™ Dithiothreitol
UltraPure Dithiothreitol (DTT, Cleland's reagent), which reduces disulfides to their corresponding thiols, is used at low concentrations to stabilize enzymes containing free sulfhydryl groups.
MilliporeSigma™ Calbiochem™ Hypoxanthine
Used in conjunction with thymidine and aminopterin for cloning and selection of hybridoma cell lines
MilliporeSigma™ Novagen™ T7·Tag™ Antibody HRP Conjugate
Single detection reagent that eliminates need for secondary antibodies or streptavidin conjugates
MilliporeSigma™ Novagen™ S·Tag™ rEK Purification Kit
Enables target protein release by incubation of the agarose beads with Recombinant Enterokinase (rEK)
MilliporeSigma™ Novagen™ Pellet Paint™ NF Co-Precipitant
Useful substitute for the original Pellet Paint Co-Precipitant in applications where fluorescent detection is used
MilliporeSigma™ Novagen™ X-Gal Solution
Convenient for blue/white screening
MilliporeSigma™ Calbiochem™ Tetrahydrouridine
Potent competitive inhibitor of cytidine deaminase
Polysorbate 80
CAS: 9005-65-6 Synonym: Polyoxyethylene(20)sorbitan monooleate
Thermo Scientific™ Co-factors
Thermo Scientific enzymes for in vitro transcription are designed for efficient and reliable RNA synthesis. These enzymes cover the entire synthesis workflow from template preparation to post-transcriptional modifications, ensuring optimal performance and high yields of synthesized RNA.